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geneatlas fluidics station 400  (Thermo Fisher)


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    Structured Review

    Thermo Fisher geneatlas fluidics station 400
    Geneatlas Fluidics Station 400, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluidics+station+400/pmc11487612-204-13-17
    Average 90 stars, based on 1 article reviews
    geneatlas fluidics station 400 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Microarray:

    Article Title: The Microglial Transcriptome of Age-Associated Deep Subcortical White Matter Lesions Suggests a Neuroprotective Response to Blood-Brain Barrier Dysfunction.
    Article Snippet: .. Using the Fluidics Station 400 (Affymetrix®, High Wycombe, UK) and GeneChip Operating System (Affymetrix®, High Wycombe, UK), a series of washing steps followed to remove any unbound DNA, before each microarray was stained and scanned using the GeneChip 3000 scanner (Affymetrix®, High Wycombe, UK). .. Microarray Data Analysis Affymetrix Expression Console software version 1.4.1.46 (Affymetrix®, High Wycombe, UK) was employed to quality control the data and principal component analysis (PCA) in Qlucore Omics Explorer software version 3.9 (Qlucore, Lund, Sweden) was used to visually inspect the data and identify sample outliers.

    Article Title: Use of biomarkers and therapeutic agents with surgical devices
    Article Snippet: Labeled RNA is hybridized to the GeneChip Porcine Genome Arrays (Affymetrix) which contain 23,937 probe sets to interrogate 23,256 transcripts in pig. .. The arrays are scanned at the Fluidics Station 400 (Affymetrix) and microarray expression data are generated with GeneChip Operating Software (GCOS; Affymetrix). ..

    Article Title: The Microglial Transcriptome of Age-Associated Deep Subcortical White Matter Lesions Suggests a Neuroprotective Response to Blood–Brain Barrier Dysfunction
    Article Snippet: .. Using the Fluidics Station 400 (Affymetrix ® , High Wycombe, UK) and GeneChip Operating System (Affymetrix ® , High Wycombe, UK), a series of washing steps followed to remove any unbound DNA, before each microarray was stained and scanned using the GeneChip 3000 scanner (Affymetrix ® , High Wycombe, UK). .. Affymetrix Expression Console software version 1.4.1.46 (Affymetrix ® , High Wycombe, UK) was employed to quality control the data and principal component analysis (PCA) in Qlucore Omics Explorer software version 3.9 (Qlucore, Lund, Sweden) was used to visually inspect the data and identify sample outliers.

    Article Title: Genome-wide analysis of pain-, nerve- and neurotrophin -related gene expression in the degenerating human annulus
    Article Snippet: Total RNA was isolated via a modified version of TRIzol Reagent (Life Technologies: Invitrogen, Carlsbad, CA, USA), and prepared for microarray hybridization using the GeneChip 3’ IVT Express Kit (Affymetrix, Santa Clara, CA). .. In brief, total RNA was reverse transcribed to synthesize cDNA, converted to double stranded DNA, subjected to transcription generating biotin-labeled amplified RNA (cRNA) and hybridized to the DNA microarray in the Affymetrix Fluidics Station 400. .. Affymetrix human U133 X3P arrays were used.

    Staining:

    Article Title: The Microglial Transcriptome of Age-Associated Deep Subcortical White Matter Lesions Suggests a Neuroprotective Response to Blood-Brain Barrier Dysfunction.
    Article Snippet: .. Using the Fluidics Station 400 (Affymetrix®, High Wycombe, UK) and GeneChip Operating System (Affymetrix®, High Wycombe, UK), a series of washing steps followed to remove any unbound DNA, before each microarray was stained and scanned using the GeneChip 3000 scanner (Affymetrix®, High Wycombe, UK). .. Microarray Data Analysis Affymetrix Expression Console software version 1.4.1.46 (Affymetrix®, High Wycombe, UK) was employed to quality control the data and principal component analysis (PCA) in Qlucore Omics Explorer software version 3.9 (Qlucore, Lund, Sweden) was used to visually inspect the data and identify sample outliers.

    Article Title: Selective Destabilization of Transcripts by mRNA Decapping Regulates Oocyte Maturation and Innate Immunity Gene Expression during Ageing in C. elegans
    Article Snippet: Following fragmentation, 15μg of cRNA were hybridized for 16 h at 45 °C on Affymetrix GeneChip C. elegans Genome Array chips. .. GeneChips were washed and stained in the Affymetrix Fluidics Station 400, following standard procedures, according to the GeneChip expression analysis technical manual. .. For the purpose of quality control, raw files were preprocessed with MAS 5.0 algorithm [ ] and the Affymetrix default Chip Description File (CDF).

    Article Title: Investigation of the molecular profile of basal cell carcinoma using whole genome microarrays
    Article Snippet: .. Arrays were subsequently washed (Affymetrix Fluidics Station 400) and stained with streptavidin-phycoerythrin (Stain Buffer, 2 mg/ml acetylated BSA and 10 μg/ml streptavidin R-phycoerythrin; Molecular Probes, Inc., Eugene, OR), and were scanned on an Affymetrix GCS GeneChip GeneArray scanner. .. Resulting data was analysed using GCOS, dCHIP, and GeneSpring (Agilent Technologies).

    Article Title: The Microglial Transcriptome of Age-Associated Deep Subcortical White Matter Lesions Suggests a Neuroprotective Response to Blood–Brain Barrier Dysfunction
    Article Snippet: .. Using the Fluidics Station 400 (Affymetrix ® , High Wycombe, UK) and GeneChip Operating System (Affymetrix ® , High Wycombe, UK), a series of washing steps followed to remove any unbound DNA, before each microarray was stained and scanned using the GeneChip 3000 scanner (Affymetrix ® , High Wycombe, UK). .. Affymetrix Expression Console software version 1.4.1.46 (Affymetrix ® , High Wycombe, UK) was employed to quality control the data and principal component analysis (PCA) in Qlucore Omics Explorer software version 3.9 (Qlucore, Lund, Sweden) was used to visually inspect the data and identify sample outliers.

    Article Title: Selective Destabilization of Transcripts by mRNA Decapping Regulates Oocyte Maturation and Innate Immunity Gene Expression during Ageing in C. elegans .
    Article Snippet: Following fragmentation, 15μg of cRNA were hybridized for 16 h at 45 ◦C on Affymetrix GeneChip C. elegans Genome Array chips. .. GeneChips were washed and stained in the Affymetrix Fluidics Station 400, following standard procedures, according to the GeneChip expression analysis technical manual. .. For the purpose of quality control, raw files were preprocessed with MAS 5.0 algorithm [16] and the Affymetrix default Chip Description File (CDF).

    Article Title: Transcriptional signature of an adult brain tumor in Drosophila
    Article Snippet: .. Washing and staining were carried out on a Fluidics station 400 (Affymetrix, MAS 5.0) using 100 mM MES, 0.1 M NaCl, 0,01 % Tween 20 as stringent wash buffer. ..

    Expressing:

    Article Title: Use of biomarkers and therapeutic agents with surgical devices
    Article Snippet: Labeled RNA is hybridized to the GeneChip Porcine Genome Arrays (Affymetrix) which contain 23,937 probe sets to interrogate 23,256 transcripts in pig. .. The arrays are scanned at the Fluidics Station 400 (Affymetrix) and microarray expression data are generated with GeneChip Operating Software (GCOS; Affymetrix). ..

    Article Title: Selective Destabilization of Transcripts by mRNA Decapping Regulates Oocyte Maturation and Innate Immunity Gene Expression during Ageing in C. elegans
    Article Snippet: Following fragmentation, 15μg of cRNA were hybridized for 16 h at 45 °C on Affymetrix GeneChip C. elegans Genome Array chips. .. GeneChips were washed and stained in the Affymetrix Fluidics Station 400, following standard procedures, according to the GeneChip expression analysis technical manual. .. For the purpose of quality control, raw files were preprocessed with MAS 5.0 algorithm [ ] and the Affymetrix default Chip Description File (CDF).

    Article Title: Selective Destabilization of Transcripts by mRNA Decapping Regulates Oocyte Maturation and Innate Immunity Gene Expression during Ageing in C. elegans .
    Article Snippet: Following fragmentation, 15μg of cRNA were hybridized for 16 h at 45 ◦C on Affymetrix GeneChip C. elegans Genome Array chips. .. GeneChips were washed and stained in the Affymetrix Fluidics Station 400, following standard procedures, according to the GeneChip expression analysis technical manual. .. For the purpose of quality control, raw files were preprocessed with MAS 5.0 algorithm [16] and the Affymetrix default Chip Description File (CDF).

    Generated:

    Article Title: Use of biomarkers and therapeutic agents with surgical devices
    Article Snippet: Labeled RNA is hybridized to the GeneChip Porcine Genome Arrays (Affymetrix) which contain 23,937 probe sets to interrogate 23,256 transcripts in pig. .. The arrays are scanned at the Fluidics Station 400 (Affymetrix) and microarray expression data are generated with GeneChip Operating Software (GCOS; Affymetrix). ..

    Software:

    Article Title: Use of biomarkers and therapeutic agents with surgical devices
    Article Snippet: Labeled RNA is hybridized to the GeneChip Porcine Genome Arrays (Affymetrix) which contain 23,937 probe sets to interrogate 23,256 transcripts in pig. .. The arrays are scanned at the Fluidics Station 400 (Affymetrix) and microarray expression data are generated with GeneChip Operating Software (GCOS; Affymetrix). ..

    Reverse Transcription:

    Article Title: Genome-wide analysis of pain-, nerve- and neurotrophin -related gene expression in the degenerating human annulus
    Article Snippet: Total RNA was isolated via a modified version of TRIzol Reagent (Life Technologies: Invitrogen, Carlsbad, CA, USA), and prepared for microarray hybridization using the GeneChip 3’ IVT Express Kit (Affymetrix, Santa Clara, CA). .. In brief, total RNA was reverse transcribed to synthesize cDNA, converted to double stranded DNA, subjected to transcription generating biotin-labeled amplified RNA (cRNA) and hybridized to the DNA microarray in the Affymetrix Fluidics Station 400. .. Affymetrix human U133 X3P arrays were used.

    Amplification:

    Article Title: Genome-wide analysis of pain-, nerve- and neurotrophin -related gene expression in the degenerating human annulus
    Article Snippet: Total RNA was isolated via a modified version of TRIzol Reagent (Life Technologies: Invitrogen, Carlsbad, CA, USA), and prepared for microarray hybridization using the GeneChip 3’ IVT Express Kit (Affymetrix, Santa Clara, CA). .. In brief, total RNA was reverse transcribed to synthesize cDNA, converted to double stranded DNA, subjected to transcription generating biotin-labeled amplified RNA (cRNA) and hybridized to the DNA microarray in the Affymetrix Fluidics Station 400. .. Affymetrix human U133 X3P arrays were used.



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